12th Ljudevit Jurak International Symposium on
Comparative Pathology
June 1-2, 2001

Jukiæ S, Talan-Hraniloviæ J, Prpiæ-Mehièiæ G, Miletiæ I, Šegoviæ S, Aniæ I. 
School of Dentistry, School of Dentistry, University of Zagreb, Zagreb, Croatia
The aim of this study was to evaluate the presence of the oestrogen receptors (OR) in the human pulp tissue and the difference of expression regarding the age, sex and period of the menstrual cycles.

Tissue samples were collected from teeth extracted due to orthodontic reasons and teeth endodonticly treated as a part of prosthetic therapy. Samples were grouped regarding the age in three groups; under twenty years, between twenty and forty, and above forty. Fifteen pulps were male (five for each group). Thirty-five were female pulps. Fifteen samples were from patients under twenty, and the same number between twenty and forty. Five pulps were from patients above forty.
First two female groups contained three subgroups regarding menstrual cycles. After fixation the samples were hematoxylin-eosin stained and immunohistochemical staining was performed using alkaline phosphatase anti-alkaline posphatase (APAAP) technique. Immunohistochemical reactivities were semiquantitatively assessed by interobserver consensus between two reviewers. Immunoreactivity was scored on an intensity scale of 0 to 3(+). Statistical analysis was performed using the evaluation of arithmetic median of population. Oestrogen receptors are common appearance in odontoblasts (1,3–1,6), and endothelial cells (1,0-1,4), while in nerve fibers are somewhat rare (0,7-1,1). They are more common in odontoblasts of females than males, while in other type of cells there is no difference regarding sex. More expressed OR were found in female group above forty. No difference was found in other age groups and regarding the menstrual cycle, except for endothelial cells, where OR were more expressed in second part of menstrual cycle. 

1.Whitaker BS, Bouquot JE. Identification and semi-quantification of estrogen and progesterone receptors in peripheral giant cell lesions of the jaws. J Periodontol 1994; 65: 280-3. 

Poster Presentation