12th Ljudevit Jurak
International Symposium on
June 1-2, 2001
|AN EVALUATION OF OESTROGEN RECEPTORS IN THE HUMAN
S, Talan-Hraniloviæ J, Prpiæ-Mehièiæ G, Miletiæ
I, Šegoviæ S, Aniæ I.
School of Dentistry, School of Dentistry, University of Zagreb, Zagreb,
|The aim of this study was to evaluate the presence of the oestrogen
receptors (OR) in the human pulp tissue and the difference of expression
regarding the age, sex and period of the menstrual cycles.
Tissue samples were collected from teeth extracted due to orthodontic
reasons and teeth endodonticly treated as a part of prosthetic therapy.
Samples were grouped regarding the age in three groups; under twenty years,
between twenty and forty, and above forty. Fifteen pulps were male (five
for each group). Thirty-five were female pulps. Fifteen samples were from
patients under twenty, and the same number between twenty and forty. Five
pulps were from patients above forty.
First two female groups contained three subgroups regarding menstrual
cycles. After fixation the samples were hematoxylin-eosin stained and immunohistochemical
staining was performed using alkaline phosphatase anti-alkaline posphatase
(APAAP) technique. Immunohistochemical reactivities were semiquantitatively
assessed by interobserver consensus between two reviewers. Immunoreactivity
was scored on an intensity scale of 0 to 3(+). Statistical analysis was
performed using the evaluation of arithmetic median of population. Oestrogen
receptors are common appearance in odontoblasts (1,3–1,6), and endothelial
cells (1,0-1,4), while in nerve fibers are somewhat rare (0,7-1,1). They
are more common in odontoblasts of females than males, while in other type
of cells there is no difference regarding sex. More expressed OR were found
in female group above forty. No difference was found in other age groups
and regarding the menstrual cycle, except for endothelial cells, where
OR were more expressed in second part of menstrual cycle.
1.Whitaker BS, Bouquot JE. Identification and semi-quantification of
estrogen and progesterone receptors in peripheral giant cell lesions of
the jaws. J Periodontol 1994; 65: 280-3.