12th Ljudevit Jurak International Symposium on
Comparative Pathology
Zagreb
June 1-2, 2001
 

POSTER PRESENTATION
GLYCOSYLATION PATTERNS OF PLACENTAL PROTEINS IN BLIGHTED OVUM
Lj. Šerman, A. Šerman*, G. Lauc**, V. Latin***, A. Miliæ**, A. Aleksandrova**, M. Vlahoviæ, D. Šerman
Department of Biology, School of Medicine, University of Zagreb
*University Hospital for Tumors, Zagreb, Croatia
**Department of Biochemistry and Molecular Biolgy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
***Department of Obstetrics and Gynecology, University of Zagreb, Croatia
Blighted ovum is a form of miscarriage, which involves  absence or disappearance of an embryo very early in the pregnancy. Because contemporary understanding of blighted ovum is not yet clear, we have demonstrated, for the first time, a glycosylation pattern  of placental proteins in this type of pregnancy failure.
Olygosaccharide branches were detected by Western-blot method using lectins: SNA and PHA-E after preliminary separation of the proteins by discontinuous SDS-PAG electrophoresis. The analysis  brings us to  the conclusion that some glycoproteins are  present with roughly equal intensities, from the eleventh till twelvth week of intrauterine development, namely GP 61, GP 52 and GP 30 in blighted ovum as well as in normal pregnancy. Beside that, we have identified a much more intensive expression of GP 74 in blighted ovum than in the normal placenta at the beginning of the eleventh week of gestation (detected by lectin PHA-E). The same result was obtained with GP 25 which was more intensive in blighted ovum than in normal placenta at the end of eleventh week (detected by  a  lectin SNA). A conclusion can be drawn that differences beetwen blighted ovum and normal placentas at the level of quantitative glycoprotein changes can be found, but not at the qualitative level.

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Poster Presentation