12th Ljudevit Jurak International Symposium on
Comparative Pathology
Zagreb
June 1-2, 2001
 

POSTER PRESENTATION
EXPRESSION OF  INTERLEUKIN 1 - AND TUMORAL NECROSIS FACTOR- BY PULMONARY INTRAVASCULAR MACROPHAGES IN ACUTE AFRICAN SWINE FEVER
F.J. Salguero, A. Núñez, C. Sanchez*, P.J. Sanchez-Cordón, J.C. Gómez-Villamandos, L. Carrasco
Department of Veterinary Pathology, Faculty of Veterinary Medicine, UCO , Rabanales,  Córdoba,Spain
*CISA-INIA Valdeolmos, Madrid, Spain
AIM OF THE STUDY: To evaluate the relationship between the apparition of pulmonary oedema and viral replication and the expression of IL-1 and TNF- by the Pulmonary Intravascular Macrophages (PIMs) in the acute form of  African Swine Fever.
MATERIALS & METHODS: 21 pigs were inoculated intramuscularly with ASFV and killed in groups of three among day 1 and 7 post inoculation. Two  animals were used as uninfected controls. Samples of lung were fixed in Bouin´s solution and glutaraldehyde (2.5%) and routinary processed for inmunohistochemical and ultrastructural studies. The inmunohistochemical study was developed  according to the ABC technique using antibodies against IL-1, TNF-, SWC-3 and viral antigen Vp73.
RESULTS: In  inoculated animals, apparition of viral replication was observed in PIMs coinciding with an intense activation of these macrophages that was accompanied by the expression of IL-1 and TNF- by these cells, and with the existence of an interstitial oedema, neutrophil secuestration and apparition of fibrin microtrombi in septal capillaries. However, while the expression of both cytokines presented a peak in the first phase of the disease, it decreased  in the following days, viral replication showed a progressive increase, reaching its peak at the final phase of the disease. 
Poster Presentation