12th Ljudevit Jurak International Symposium on
Comparative Pathology
Zagreb
June 1-2, 2001
 

POSTER PRESENTATION
SUBARACHNOID HEMORRHAGE IN RABBITS - A  COMPUTER IMAGE ANALYSIS AND DBH IMMUNOREACTIVITY - Results of a Preliminary Study
S. Kovaèiæ, G. Bunc
Teaching Hospital, Maribor, Slovenia
The effect of sympathetic exclusion of vasospasm and DBH immunoreactivity of central adrenergic neurons was studied in a series of rabbits under conditions imitating human subarachnoid hemorrhage. The single hemorrhage model of experimental subarachnoid was chosen. The study design consisted of four groups of female New Zealand rabbits with an average weight of 3 kg. The control group A comprised of rabbits without subarachnoid hemorrhage, group B with subarachnoid hemorrhage, group C with subarachnoid hemorrhage and medicamentous sympathetic exclusion by perorally administered alpha blocker, and group D with subarachnoid hemorrhage and an operative sympathetic exclusion by cervical gangliectomy. Comparison of the arterial intima corrugation showed that exclusion of the sympathetic nervous system prevents vasospasm of the cerebral arteries after experimental subarachnoid hemorrhage in rabbits. Changes were evaluated by computer image analysis. Dopamine beta hydroxylase (DBH) is the enzyme that converts dopamine to norephinephrine. It could serve as a convenient marker for the study of noradrenergic cell functions. Degree of immunoreactivity of the central adrenergic neurons in  the mesencephalon and locus ceruleus was evaluated by staining  tissue sections with the anti-dopamine beta hydroxylase monoclonal antibody. Comparison of the tissue sections shows different intensities of immunoreactivity between examinated groups. The results of this study suggest a decreased DBH level in the sympathetic nervous system by the exclusion of the sympathetic nervous systems activity. Exclusion of the sympathetic system envisages a significant influence to prevent vasospasm in rabbits.
Poster Presentation