12th Ljudevit Jurak International Symposium on
Comparative Pathology
Zagreb
June 1-2, 2001
 

POSTER PRESENTATION
QUANTITATIVE AND QUALITATIVE COLLAGEN CONTENT IN THE MUSCLE OF CHICKEN FED WITH DIETS SUPPLEMENTED BY AN INORGANIC BIOELEMENTS COMPOUND ”MICROSOL” (Fe, Cu, Mn, Zn, AND Co)
D. Curcæ, V. Andronie, C.I. Andronie
Department of Physiopathology, Faculty of Veterinary Medicine, Bucharest, Romania
Collagen is a scleroproteid similar to the two components of the conjunctive tissue: elastine and reticuline. Collagen represents 15-50% from the total conjunctive tissue and 30-35% of the total protein in the body.
Quantitative and qualitative modifications are highly important in the understanding of different biologic processes such as growth and development or pathologic processes with an implication to this protein, which is spread trought the body.
The determination of the muscles collagen content within the chicken feed with diets supplemented by the inorganic bioelemental compounds from ”Microsol” (Fe, Cu, Mn, Zn and Co) was made after 1 month of supplementation.
Collagen dosing was carried out by an indirect biochemical method, which considers hydroxyproline dosing in accordance with the method described by Neuman and Logan. After the reading of extinctions using the standard curve, the contents of hydroxyproline in the samples are measured calculating the quantity of hydroxyproline in mg in 100 g muscle. The transformation of hydroxyproline into collagen is done by multiplicating it with the factor 7.25 by Goll and al. method. Collagen quality was evaluated by the Hill’s method, based on  determining of collagen solubility in ¼ of warm Ringer solution.
After administrating of one month broiler chickens feeding on 100 ml Microsol/10 kg mixed feed stuff, diet 21/l for 30 days, we found out an improvement in the maintenance and a good natural mixture of fat and flesh, the contents of collagen increased in comparison with chickens in the control batch. As a result, in the inside of pectoral muscles there was a 20.25% rise due to the increase of soluble collagen contents, which increased only 18.79% and the ratio between insoluble and soluble collagen was 0,60. The same tendency of increasing collagen has also been noted in the muscle of the thigh-shank were the total collagen increased with 14.26% due to an important rise of soluble collagen, which was 31.32% in comparison with the control batch while the insoluble collagen hasn’t shown any important variations and thus not taken into account. The ratio between insoluble/soluble collagen was 0.88 while in the control batch it was 1.16, which represents a decrease of 24.15%.
In the second variant, a quantity of 200 ml Microsol/10 kg of mixed feed stuff was administrated combined with the diet 21/1. After 30 days feeding of with the feed stuff supplemented containing bioelements, the total collagen from the pectoral muscles increased with 34.24% in comparison with the control batch. This increase was carried out considering the contents of insoluble collagen, which increased with 113.25% while soluble collagen decreased with 14.22%. The ratio between insoluble/soluble collagen increased from 0.61 in the control batch to 1.52 in the chickens from the batch that was supplemented with 200 ml Microsol/10 kg mixed feed stuff.

Acknowledgements
The research field was supported by the Grant-in-Aid for Ministry of Education, Romania, No. CNCSIS 691/2000, Theme No. 3 in the fiscal year 2000.

Poster Presentation