12th Ljudevit Jurak
International Symposium on
June 1-2, 2001
|QUANTITATIVE AND QUALITATIVE COLLAGEN CONTENT
IN THE MUSCLE OF CHICKEN FED WITH DIETS SUPPLEMENTED BY AN INORGANIC BIOELEMENTS
COMPOUND ”MICROSOL” (Fe, Cu, Mn, Zn, AND Co)
|D. Curcæ, V. Andronie, C.I. Andronie
Department of Physiopathology, Faculty of Veterinary Medicine, Bucharest,
|Collagen is a scleroproteid similar to the two components of the conjunctive
tissue: elastine and reticuline. Collagen represents 15-50% from the total
conjunctive tissue and 30-35% of the total protein in the body.
Quantitative and qualitative modifications are highly important in
the understanding of different biologic processes such as growth and development
or pathologic processes with an implication to this protein, which is spread
trought the body.
The determination of the muscles collagen content within the chicken
feed with diets supplemented by the inorganic bioelemental compounds from
”Microsol” (Fe, Cu, Mn, Zn and Co) was made after 1 month of supplementation.
Collagen dosing was carried out by an indirect biochemical method,
which considers hydroxyproline dosing in accordance with the method described
by Neuman and Logan. After the reading of extinctions using the standard
curve, the contents of hydroxyproline in the samples are measured calculating
the quantity of hydroxyproline in mg in 100 g muscle. The transformation
of hydroxyproline into collagen is done by multiplicating it with the factor
7.25 by Goll and al. method. Collagen quality was evaluated by the Hill’s
method, based on determining of collagen solubility in ¼
of warm Ringer solution.
After administrating of one month broiler chickens feeding on 100 ml
Microsol/10 kg mixed feed stuff, diet 21/l for 30 days, we found out an
improvement in the maintenance and a good natural mixture of fat and flesh,
the contents of collagen increased in comparison with chickens in the control
batch. As a result, in the inside of pectoral muscles there was a 20.25%
rise due to the increase of soluble collagen contents, which increased
only 18.79% and the ratio between insoluble and soluble collagen was 0,60.
The same tendency of increasing collagen has also been noted in the muscle
of the thigh-shank were the total collagen increased with 14.26% due to
an important rise of soluble collagen, which was 31.32% in comparison with
the control batch while the insoluble collagen hasn’t shown any important
variations and thus not taken into account. The ratio between insoluble/soluble
collagen was 0.88 while in the control batch it was 1.16, which represents
a decrease of 24.15%.
In the second variant, a quantity of 200 ml Microsol/10 kg of mixed
feed stuff was administrated combined with the diet 21/1. After 30 days
feeding of with the feed stuff supplemented containing bioelements, the
total collagen from the pectoral muscles increased with 34.24% in comparison
with the control batch. This increase was carried out considering the contents
of insoluble collagen, which increased with 113.25% while soluble collagen
decreased with 14.22%. The ratio between insoluble/soluble collagen increased
from 0.61 in the control batch to 1.52 in the chickens from the batch that
was supplemented with 200 ml Microsol/10 kg mixed feed stuff.
The research field was supported by the Grant-in-Aid for Ministry of
Education, Romania, No. CNCSIS 691/2000, Theme No. 3 in the fiscal year