12th Ljudevit Jurak
International Symposium on
June 1-2, 2001
|TESTICULAR MACROPHAGES AND STEREOLOGICAL CHARACTERISTICS
OF SEMINIFEROUS TUBULES OF INFERTILE MEN
D. Ježek, D. Kalafatiæ, Lj. Banek, M. Petanjek, I. Krhen
Medical School University of Zagreb,
|AIMS: 1. to determine and to compare testicular volume, status of spermatogenesis
(SCORE), length and curvature of seminiferous tubules and number of testicular
macrophages in fertile and infertile men; 2. to establish if there is an
intercorrelation between this data.
METHODS: Testicular biopsies were divided into a control (10 men who
underwent a vasectomy reversal) and an infertile group (45 patients with
idiopathic infertility and azoospermia). The testicular volume was determined
by an orchidometer and an ultrasound examination in both groups. The status
of spermatogenesis was estimated by Johnsen’s modified score system. Applying
Weibel’s multipurpose test system with 42 points, length and curvature
of the seminiferous tubules and the number and volume of testicular macrophages
were calculated. The immunohistochemical and morphometric analysis of macrophages
(the expression of KiM1P antigen in cells) was done, and the correlation
coefficient of all analysed variables was determined.
RESULTS: Infertile testis had significantly decreased volume and a
lower score (status of spermatogenesis) when compared to controls. Testicular
tissue of infertile patients has shown significant patohistological changes:
the interstitial tissue was sporadically fibrosed and contained much more
macrophages; the seminiferous tubules had decreased in diameter; they had
seminiferous epithelium in various degrees of degenerative changes;
in some places seminiferous epitehlium was infiltrated with macrophages.
The length and curvature of seminiferous tubules per mm3 of the testis
tissue were significantly increased in the infertile group, while
the same variables calculated for the entire testis were significantly
decreased. The number of macrophages analysed for mm3 of testis tissue
was significantly increased. The same variable analysed for the entire
testis was significantly reduced. The volume of macrophages in mm3 of testis
tissue was significantly larger in the group of infertile men, while the
volume of macrophages in the whole testis did not show a significant difference
between the compared groups. The results of correlation analysis of the
examined variables indicate a significant connection of the relative values
(macrophages number and volume) with stereological characteristics of testis
expressed in mm3 of tissue.
CONCLUSION: The population of testicular macrophages of infertile men
differs both qualitatively and quantitatively from the macrophages in the
testis with a preserved structure and reproductive ability.
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