12th Ljudevit Jurak International Symposium on
Comparative Pathology
Zagreb
June 1-2, 2001
POSTER PRESENTATION
DISTRIBUTION OF CYTOKERATIN ON THE OESOPHAGOGASTRICAL REGION IN SWINE GASTERS WITH DIFFERENT DEGREES OF LESIONS 
T. Ristoski, Milijana Kneževiæ*, Sanja Kovaèeviæ*
Department of Pathology, Faculty of Veterinary Medicine, Skopje, Macedonia
*Department of Pathology, Faculty of Veterinary Medicine, Belgrade, Yugoslavia
Histopathological and immunohistochemical examinations of tissue sections from swine gasters with lesions on the oesophagogastical region were the subject of this study.
Base on the intensity of the lesions of pars oesophagea, gasters were divided in three groups:
1) Six gasters with hyperkeratosis,
2) Six gasters with hyperkeratosis and erosion and
3) Control group - six gasters without morphological changes .
For the immunohistochemical examinations the direct peroxidasis (DP) method was applied. To detect a cytokeratin expression in the three groups with different histopathological lesions the Monoclonal mouse anti human Cytokeratin/HRP (Dako,U7022) was used. The most prominent expression of cytokeratin was observed on the basal and suprabasal layers of pars esophagea epithelium. The immunohistochemical reaction of cytokeratin was cytoplasmatic. Cells with parakeratosis and a separated hyperkeratotic layers on the surface of the epithelium were cytokeratin negative. Expression of cytokeratin in the basal and suprabasal layers in the gaster with hyperkeratosis was considerably increased. Endothelial cells of some blood vessels and epithelium of the cardiac glands were cytokeratin positive, while lymphatic tissue was cytokeratin negative.
Key words: Cytokeratin, hyperkeratosis, erosions, gaster, swine
Poster Presentation

 
 

References:
Argenzio R.A., Eisemann J. (1996) Mechanismus of acid injury in porcine gastroesophageal mucosa. Am. Vet. Res. 4, 564-573.
J.H. Vos et al., (1992) Immunohistochemistry with keratin and smooth muscle actin monoclonal antibodies in canine digestive tract and extramural glands. Journal of Veterinary Madicine, ,  A 39, 241-257.
J.H. Vos et al., (1989) Keratin staining of canine epithelial tissues by a polyclonal antiserum. Journal of Veterinary Madicine A 36, 374-385.
Jubb K.V.F., Kennedy P.C., Palmer N, 1993: Pathology of Domestic Animals, 4th ed. New York: Academic Press, Inc.
Querioz D.M. et al., (1996) Association between Helicobacter and gastric ulcer disease of the pars esophagea in swine. Gastroenterology 1, 19-27.
Roels S., Ducatelle R., Broekaert D. (1997) Keratin pattern in hyperceratotic and ulcerated gastric pars oesophagea in pigs. Res. Vet. Sci., 2, 165-169