12th Ljudevit Jurak
International Symposium on
June 1-2, 2001
|DISTRIBUTION OF CYTOKERATIN ON THE OESOPHAGOGASTRICAL
REGION IN SWINE GASTERS WITH DIFFERENT DEGREES OF LESIONS
|T. Ristoski, Milijana Kneževiæ*, Sanja
Department of Pathology, Faculty of Veterinary Medicine, Skopje, Macedonia
*Department of Pathology, Faculty of Veterinary Medicine, Belgrade,
|Histopathological and immunohistochemical examinations of tissue sections
from swine gasters with lesions on the oesophagogastical region were the
subject of this study.
Base on the intensity of the lesions of pars oesophagea, gasters were
divided in three groups:
1) Six gasters with hyperkeratosis,
2) Six gasters with hyperkeratosis and erosion and
3) Control group - six gasters without morphological changes .
For the immunohistochemical examinations the direct peroxidasis (DP)
method was applied. To detect a cytokeratin expression in the three groups
with different histopathological lesions the Monoclonal mouse anti human
Cytokeratin/HRP (Dako,U7022) was used. The most prominent expression of
cytokeratin was observed on the basal and suprabasal layers of pars esophagea
epithelium. The immunohistochemical reaction of cytokeratin was cytoplasmatic.
Cells with parakeratosis and a separated hyperkeratotic layers on the surface
of the epithelium were cytokeratin negative. Expression of cytokeratin
in the basal and suprabasal layers in the gaster with hyperkeratosis was
considerably increased. Endothelial cells of some blood vessels and epithelium
of the cardiac glands were cytokeratin positive, while lymphatic tissue
was cytokeratin negative.
Key words: Cytokeratin, hyperkeratosis, erosions, gaster,
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