12th Ljudevit Jurak International Symposium on
Comparative Pathology
Zagreb
June 1-2, 2001
 

ABSTRACT
THE INHIBITORY EFFECT OF ALCOHOL ON 1,2 -DIMETHYHYDRAZINE INDUCED INTESTINAL TUMORS IN WISTAR RAT
A. Zebiè, A. Cerar, D. Pokorn*, C. Hlastan-Ribiè*
Medical Experimental Center, Institute of Pathology, *Institute of Hygiene, Medical Faculty, Ljubljana, Slovenia
Introduction: Some epidemiological studies within the human population have shown an association between consumption of alcoholic beverages and the increased occurrence of large bowel carcinoma. Many studies on experimental models of colonic carcinogenesis have not shown significant correlation between alcohol consumption and the incidence of intestinal tumors.
Aim of the study: In our study effects of 11% ethanol and wine (8,5% ethanol) were assessed in the rat - 1,2-dimethylhydrazine (DMH) experimental intestinal carcinogenesis model.
Methods and materials: Eighty-seven 10-Wk old outbred male Wistar rats were distributed into three groups (control, 11% ethanol and wine group). DMH was administrated once a Wk. s.c. for 15 Wk. (20mg/kg bw). After six months the experiment was terminated and complete necropsy was performed. Intestines and other macroscopically changed organs were examined histologically.
Results: In the control group we found 13 adenocarcinomas (3 in the jejunum, 10 in the colon), 2 adenomas (in the colon) and 1 adenocarcinoma of Zymbal gland. In the ethanol group we found 3 adenocarcinomas (1 in the jejunum, 2 in the colon) and 1 adenoma (in the colon.). In the wine group there were 3 adenocarcinomas (in the colon) and 2 adenomas (in the colon). The control group had significantly more adenocarcinomas than that of the experimental groups (P < 0,01), the difference in the
incidence of adenomas was not significant (P > 0,5). Also there was no significant difference in the tumor incidence between the experimental groups (P > 0,5).
Conclusion: Our study showed the inhibitory effect of ethanol and wine on the development of intestinal tumours. The ethanol group as well as the wine group in our experiment had the same number of tumors, and therefore we assume, that the inhibitory effect on intestinal carcinogenesis is the result of ethanol ingestion and its protective action on the mucosa and not that of wine’s nonethanol components.
Program