12th Ljudevit Jurak
International Symposium on
June 1-2, 2001
|THE INHIBITORY EFFECT OF ALCOHOL ON 1,2 -DIMETHYHYDRAZINE
INDUCED INTESTINAL TUMORS IN WISTAR RAT
A. Cerar, D. Pokorn*, C. Hlastan-Ribiè*
Medical Experimental Center, Institute of Pathology, *Institute of
Hygiene, Medical Faculty, Ljubljana, Slovenia
|Introduction: Some epidemiological studies within the human population
have shown an association between consumption of alcoholic beverages and
the increased occurrence of large bowel carcinoma. Many studies on experimental
models of colonic carcinogenesis have not shown significant correlation
between alcohol consumption and the incidence of intestinal tumors.
Aim of the study: In our study effects of 11% ethanol and wine (8,5%
ethanol) were assessed in the rat - 1,2-dimethylhydrazine (DMH) experimental
intestinal carcinogenesis model.
Methods and materials: Eighty-seven 10-Wk old outbred male Wistar rats
were distributed into three groups (control, 11% ethanol and wine group).
DMH was administrated once a Wk. s.c. for 15 Wk. (20mg/kg bw). After six
months the experiment was terminated and complete necropsy was performed.
Intestines and other macroscopically changed organs were examined histologically.
Results: In the control group we found 13 adenocarcinomas (3 in the
jejunum, 10 in the colon), 2 adenomas (in the colon) and 1 adenocarcinoma
of Zymbal gland. In the ethanol group we found 3 adenocarcinomas (1 in
the jejunum, 2 in the colon) and 1 adenoma (in the colon.). In the wine
group there were 3 adenocarcinomas (in the colon) and 2 adenomas (in the
colon). The control group had significantly more adenocarcinomas than that
of the experimental groups (P < 0,01), the difference in the
incidence of adenomas was not significant (P > 0,5). Also there was
no significant difference in the tumor incidence between the experimental
groups (P > 0,5).
Conclusion: Our study showed the inhibitory effect of ethanol and wine
on the development of intestinal tumours. The ethanol group as well as
the wine group in our experiment had the same number of tumors, and therefore
we assume, that the inhibitory effect on intestinal carcinogenesis is the
result of ethanol ingestion and its protective action on the mucosa and
not that of wine’s nonethanol components.