12th Ljudevit Jurak
International Symposium on
June 1-2, 2001
|ASCORBINEMIA AND SERUM CHOLINESTERASE ACTIVITY
IN PIGLETS AFTER PARENTERAL ADMINISTRATION OF ASCORBIC ACID
|D. Curcæ, V. Andronie, M. Codreanu
Department of Physiopathology, Faculty of Veterinary Medicine, Bucharest,
|The importance of ascorbic acid for domestic animals is proved by following
benefits: it promotes healthy capillaries, aids iron absorption, helps
heal wounds and broken bones, treats anemia (especially for iron-deficiency
anemia), treats urinary tract infections, helps form collagen in connective
tissue, increases iron absorption in the intestines, contributes to hemoglobin
and red blood cell production in bone marrow, blocks production of nitrosamines.
Ascorbic acid and its interaction with other substances: anti-cholinergics
decrease anti-cholinergic effect; anti-coagulants (oral) decrease
anti-coagulant effect; aspirin and barbiturates decrease ascorbic acid
(vitamin C) effect; iron supplements increase iron effect.
Animals of different age and mainly piglets are reported to be
able to synthetize a sufficient amount of ascorbic acid from glucose in
their gut in order to satisfy their requirements. However, it has been
suggested that the ascorbic acid requirement was increased during the reproductive
period. More recently dietary supplementation of ascorbic acid was found
to exert different effects on the growth rate of weanling and fattening
Young animals seemed to be more sensitive to supplementation during
particular periods such as weaning. In fact, large variations observed
between experiments could be associated with the experimental conditions
such as: age of the animals or the period of life, length of the experiment,
housing conditions, level of supply and stability of the ascorbic acid
in the diet.
A study was made regarding the total blood level of ascorbic acid (AA)
using the Roe and Kuether method; and of serum cholinesterase activity
(PChE) using the Ellman et al. method, in hypothrepsic piglets, after parenteral
administration of ascorbic acid, as compared with untreated piglets and
with clinically healthy congeners. Ascorbic acid, 10% solution, was given
i.m. 0,25 g/day for 4 succesive days to hypothrepsic piglets at birth,
10 days and at 20 days after birth.
Following parenteral administration of AA a 129.44% rise in ascorbinemia
was found in the treated piglets against their clinically healthy congeners,
and a 320,98% rise against the untreated hypothrepsic piglets. Serum cholinesterase
activity rose 14.47% against the untreated hypothrepsic piglets.
After the administration of AA a 232.83% rise in ascorbinemia was found
in 14 day old hypothrepsic piglets against the clinically healthy congeners,
and a 180.49% rise against the untreated hypothrepsic piglets. Serum cholinesterase
activity dropped 21.22% against the untreated hypothrepsic piglets, and
37.80% against the clinically healthy congeners.
In 24-day old hypothrepsic piglets given AA a 165.16% rise in
ascorbinemia was found against the untreated hypothrepsic piglets and a
120.39% rise against the clinically healthy congeners. Serum cholinesterase
activity was 31.58% lower against the untreated hypothrepsic piglets and
25.53% lower against the clinically healthy congeners.
The results also reveal the existence of a relatively wide variation
of ascorbinemia and serum cholinesterase activity in suckling piglets,
depending on age, state of health, and parenteral administration of AA.